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elavl3  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology elavl3
    (A) Schematic timeline of brain organoids generation from mESCs. mESCs and brain organoids developed for 7, 14 and 21 days were profiled by transcriptomics and proteomics. Part of the figure was created with Biorender. (B) Immunostaining of mESCs-derived brain organoids. Cultures of mESCs were stained with NANOG (green), POU5F1/OCT4 (red), and nuclei with DAPI (blue). Scale bars: 10 µm. (C) Organoid cryosections were stained with NESTIN and PAX6 (at day 7), <t>ELAVL3</t> and TBR1 (at day 14), and TUBB3 and GFAP (at day 21). Scale bars: 100 µm. (D) PCA showing the distribution of the transcriptomes of mESCs, brain organoids (at days 7, 14, and 21), and NBB samples. (E) Venn diagram showing the overlapping upregulated and downregulated genes between D21 organoids and NBB, both compared to mESCs. (F) Histograms of enriched GO terms in NBB vs ESCs and D21 organoids vs ESCs performed on upregulated genes (black and grey bars) and on downregulated genes (red and light red bars) determined using DAVID. For each term category (Cellular component, molecular function, and biological process), the five highest enriched terms in each gene list are shown.
    Elavl3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/elavl3/HuC+Antibody/bio_rxiv__64898__2026__03__13__711293-190-27-29
    Average 93 stars, based on 27 article reviews
    elavl3 - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "The RNA and protein landscapes of mouse brain organoids"

    Article Title: The RNA and protein landscapes of mouse brain organoids

    Journal: bioRxiv

    doi: 10.64898/2026.03.13.711293

    (A) Schematic timeline of brain organoids generation from mESCs. mESCs and brain organoids developed for 7, 14 and 21 days were profiled by transcriptomics and proteomics. Part of the figure was created with Biorender. (B) Immunostaining of mESCs-derived brain organoids. Cultures of mESCs were stained with NANOG (green), POU5F1/OCT4 (red), and nuclei with DAPI (blue). Scale bars: 10 µm. (C) Organoid cryosections were stained with NESTIN and PAX6 (at day 7), ELAVL3 and TBR1 (at day 14), and TUBB3 and GFAP (at day 21). Scale bars: 100 µm. (D) PCA showing the distribution of the transcriptomes of mESCs, brain organoids (at days 7, 14, and 21), and NBB samples. (E) Venn diagram showing the overlapping upregulated and downregulated genes between D21 organoids and NBB, both compared to mESCs. (F) Histograms of enriched GO terms in NBB vs ESCs and D21 organoids vs ESCs performed on upregulated genes (black and grey bars) and on downregulated genes (red and light red bars) determined using DAVID. For each term category (Cellular component, molecular function, and biological process), the five highest enriched terms in each gene list are shown.
    Figure Legend Snippet: (A) Schematic timeline of brain organoids generation from mESCs. mESCs and brain organoids developed for 7, 14 and 21 days were profiled by transcriptomics and proteomics. Part of the figure was created with Biorender. (B) Immunostaining of mESCs-derived brain organoids. Cultures of mESCs were stained with NANOG (green), POU5F1/OCT4 (red), and nuclei with DAPI (blue). Scale bars: 10 µm. (C) Organoid cryosections were stained with NESTIN and PAX6 (at day 7), ELAVL3 and TBR1 (at day 14), and TUBB3 and GFAP (at day 21). Scale bars: 100 µm. (D) PCA showing the distribution of the transcriptomes of mESCs, brain organoids (at days 7, 14, and 21), and NBB samples. (E) Venn diagram showing the overlapping upregulated and downregulated genes between D21 organoids and NBB, both compared to mESCs. (F) Histograms of enriched GO terms in NBB vs ESCs and D21 organoids vs ESCs performed on upregulated genes (black and grey bars) and on downregulated genes (red and light red bars) determined using DAVID. For each term category (Cellular component, molecular function, and biological process), the five highest enriched terms in each gene list are shown.

    Techniques Used: Transcriptomics, Immunostaining, Derivative Assay, Staining

    Related Articles

    Transcriptomics:

    Article Title: The RNA and protein landscapes of mouse brain organoids
    Article Snippet: Primary antibodies were incubated overnight at 4°C.Primary antibodies were incubated overnight at 4°C.. The primary antibodies were (species, provider; catalog number): anti-NESTIN –Rat-401- (mouse, Santa-Cruz, sc-33677); PAX6 (Rabbit, Covance, PRB-278P); NANOG (mouse, BD Pharmingen, 560259); POU5F1 (rabbit, Cell Signalling, 2840), ELAVL3 (mouse, Santa Cruz, sc-515624), TUBB3 (mouse, Covance, MMS-435P); TBR1 (rabbit, Cell Signalling, 49661), REELIN (mouse, Covance; MAB5364), GRM5 (rabbit, Millipore, AB5675), and GFAP (rabbit, Dako, Z0334).. Cryosections were washed three times with PBS and incubated with secondary antibodies (anti-mouse or anti-rabbit coupled to Cy2 or Cy3 with minimal cross-species reactivity, Jackson Immunoresearch) for 2h at RT, both diluted in PBS containing 0.1% Triton X-100 and 4% FBS.Cryosections were washed three times with PBS and incubated with secondary antibodies (anti-mouse or anti-rabbit coupled to Cy2 or Cy3 with minimal cross-species reactivity, Jackson Immunoresearch) for 2h at RT, both diluted in PBS containing 0.1%..

    Immunostaining:

    Article Title: The RNA and protein landscapes of mouse brain organoids
    Article Snippet: Primary antibodies were incubated overnight at 4°C.Primary antibodies were incubated overnight at 4°C.. The primary antibodies were (species, provider; catalog number): anti-NESTIN –Rat-401- (mouse, Santa-Cruz, sc-33677); PAX6 (Rabbit, Covance, PRB-278P); NANOG (mouse, BD Pharmingen, 560259); POU5F1 (rabbit, Cell Signalling, 2840), ELAVL3 (mouse, Santa Cruz, sc-515624), TUBB3 (mouse, Covance, MMS-435P); TBR1 (rabbit, Cell Signalling, 49661), REELIN (mouse, Covance; MAB5364), GRM5 (rabbit, Millipore, AB5675), and GFAP (rabbit, Dako, Z0334).. Cryosections were washed three times with PBS and incubated with secondary antibodies (anti-mouse or anti-rabbit coupled to Cy2 or Cy3 with minimal cross-species reactivity, Jackson Immunoresearch) for 2h at RT, both diluted in PBS containing 0.1% Triton X-100 and 4% FBS.Cryosections were washed three times with PBS and incubated with secondary antibodies (anti-mouse or anti-rabbit coupled to Cy2 or Cy3 with minimal cross-species reactivity, Jackson Immunoresearch) for 2h at RT, both diluted in PBS containing 0.1%..

    Derivative Assay:

    Article Title: The RNA and protein landscapes of mouse brain organoids
    Article Snippet: Primary antibodies were incubated overnight at 4°C.Primary antibodies were incubated overnight at 4°C.. The primary antibodies were (species, provider; catalog number): anti-NESTIN –Rat-401- (mouse, Santa-Cruz, sc-33677); PAX6 (Rabbit, Covance, PRB-278P); NANOG (mouse, BD Pharmingen, 560259); POU5F1 (rabbit, Cell Signalling, 2840), ELAVL3 (mouse, Santa Cruz, sc-515624), TUBB3 (mouse, Covance, MMS-435P); TBR1 (rabbit, Cell Signalling, 49661), REELIN (mouse, Covance; MAB5364), GRM5 (rabbit, Millipore, AB5675), and GFAP (rabbit, Dako, Z0334).. Cryosections were washed three times with PBS and incubated with secondary antibodies (anti-mouse or anti-rabbit coupled to Cy2 or Cy3 with minimal cross-species reactivity, Jackson Immunoresearch) for 2h at RT, both diluted in PBS containing 0.1% Triton X-100 and 4% FBS.Cryosections were washed three times with PBS and incubated with secondary antibodies (anti-mouse or anti-rabbit coupled to Cy2 or Cy3 with minimal cross-species reactivity, Jackson Immunoresearch) for 2h at RT, both diluted in PBS containing 0.1%..

    Staining:

    Article Title: The RNA and protein landscapes of mouse brain organoids
    Article Snippet: Primary antibodies were incubated overnight at 4°C.Primary antibodies were incubated overnight at 4°C.. The primary antibodies were (species, provider; catalog number): anti-NESTIN –Rat-401- (mouse, Santa-Cruz, sc-33677); PAX6 (Rabbit, Covance, PRB-278P); NANOG (mouse, BD Pharmingen, 560259); POU5F1 (rabbit, Cell Signalling, 2840), ELAVL3 (mouse, Santa Cruz, sc-515624), TUBB3 (mouse, Covance, MMS-435P); TBR1 (rabbit, Cell Signalling, 49661), REELIN (mouse, Covance; MAB5364), GRM5 (rabbit, Millipore, AB5675), and GFAP (rabbit, Dako, Z0334).. Cryosections were washed three times with PBS and incubated with secondary antibodies (anti-mouse or anti-rabbit coupled to Cy2 or Cy3 with minimal cross-species reactivity, Jackson Immunoresearch) for 2h at RT, both diluted in PBS containing 0.1% Triton X-100 and 4% FBS.Cryosections were washed three times with PBS and incubated with secondary antibodies (anti-mouse or anti-rabbit coupled to Cy2 or Cy3 with minimal cross-species reactivity, Jackson Immunoresearch) for 2h at RT, both diluted in PBS containing 0.1%..



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    Image Search Results


    (A) Schematic timeline of brain organoids generation from mESCs. mESCs and brain organoids developed for 7, 14 and 21 days were profiled by transcriptomics and proteomics. Part of the figure was created with Biorender. (B) Immunostaining of mESCs-derived brain organoids. Cultures of mESCs were stained with NANOG (green), POU5F1/OCT4 (red), and nuclei with DAPI (blue). Scale bars: 10 µm. (C) Organoid cryosections were stained with NESTIN and PAX6 (at day 7), ELAVL3 and TBR1 (at day 14), and TUBB3 and GFAP (at day 21). Scale bars: 100 µm. (D) PCA showing the distribution of the transcriptomes of mESCs, brain organoids (at days 7, 14, and 21), and NBB samples. (E) Venn diagram showing the overlapping upregulated and downregulated genes between D21 organoids and NBB, both compared to mESCs. (F) Histograms of enriched GO terms in NBB vs ESCs and D21 organoids vs ESCs performed on upregulated genes (black and grey bars) and on downregulated genes (red and light red bars) determined using DAVID. For each term category (Cellular component, molecular function, and biological process), the five highest enriched terms in each gene list are shown.

    Journal: bioRxiv

    Article Title: The RNA and protein landscapes of mouse brain organoids

    doi: 10.64898/2026.03.13.711293

    Figure Lengend Snippet: (A) Schematic timeline of brain organoids generation from mESCs. mESCs and brain organoids developed for 7, 14 and 21 days were profiled by transcriptomics and proteomics. Part of the figure was created with Biorender. (B) Immunostaining of mESCs-derived brain organoids. Cultures of mESCs were stained with NANOG (green), POU5F1/OCT4 (red), and nuclei with DAPI (blue). Scale bars: 10 µm. (C) Organoid cryosections were stained with NESTIN and PAX6 (at day 7), ELAVL3 and TBR1 (at day 14), and TUBB3 and GFAP (at day 21). Scale bars: 100 µm. (D) PCA showing the distribution of the transcriptomes of mESCs, brain organoids (at days 7, 14, and 21), and NBB samples. (E) Venn diagram showing the overlapping upregulated and downregulated genes between D21 organoids and NBB, both compared to mESCs. (F) Histograms of enriched GO terms in NBB vs ESCs and D21 organoids vs ESCs performed on upregulated genes (black and grey bars) and on downregulated genes (red and light red bars) determined using DAVID. For each term category (Cellular component, molecular function, and biological process), the five highest enriched terms in each gene list are shown.

    Article Snippet: The primary antibodies were (species, provider; catalog number): anti-NESTIN –Rat-401- (mouse, Santa-Cruz, sc-33677); PAX6 (Rabbit, Covance, PRB-278P); NANOG (mouse, BD Pharmingen, 560259); POU5F1 (rabbit, Cell Signalling, 2840), ELAVL3 (mouse, Santa Cruz, sc-515624), TUBB3 (mouse, Covance, MMS-435P); TBR1 (rabbit, Cell Signalling, 49661), REELIN (mouse, Covance; MAB5364), GRM5 (rabbit, Millipore, AB5675), and GFAP (rabbit, Dako, Z0334).

    Techniques: Transcriptomics, Immunostaining, Derivative Assay, Staining

    Pan-neuronal overexpression of c3 in the sspo ( dmh4/+ ) IS model leads to a female-specific increase in spinal curve severity. (A) A Tg( elavl3::c3 ) transgene driving pan-neuronal overexpression of c3 was cloned, and (B) expression levels in stable transgenic animals validated by qRT-PCR. (C) Tg( elavl3::c3 ) expression does not cause axial phenotypes in a wildtype background. (D) Quantification of scoliosis penetrance in sspo ( dmh4/+ ) and sspo ( dmh4/+ ); Tg( elavl3::c3 ) siblings revealed no significant difference between cohorts (Two-sided Fisher’s exact test, p=0.6346; if separated by sex: females, p>0.9999; males, p>0.9999). To quantify the effect of c3 overexpression on sspo ( dmh4/+ ) scoliosis severity in male (E) and female (I) fish, uCT imaging was performed, followed by Cobb angle analysis (F-H, J-L). The scoliosis phenotype was not penetrant in 4 fish (Tg-male, n=1; Tg+ male, n=2; Tg+ female, n=1). These data points have been eliminated from the analysis of scoliosis severity. Scale bars = 5mm.

    Journal: bioRxiv

    Article Title: Pre-clinical models of idiopathic scoliosis implicate sex-specific roles for complement activity in modulating spinal curve severity

    doi: 10.64898/2026.02.20.707049

    Figure Lengend Snippet: Pan-neuronal overexpression of c3 in the sspo ( dmh4/+ ) IS model leads to a female-specific increase in spinal curve severity. (A) A Tg( elavl3::c3 ) transgene driving pan-neuronal overexpression of c3 was cloned, and (B) expression levels in stable transgenic animals validated by qRT-PCR. (C) Tg( elavl3::c3 ) expression does not cause axial phenotypes in a wildtype background. (D) Quantification of scoliosis penetrance in sspo ( dmh4/+ ) and sspo ( dmh4/+ ); Tg( elavl3::c3 ) siblings revealed no significant difference between cohorts (Two-sided Fisher’s exact test, p=0.6346; if separated by sex: females, p>0.9999; males, p>0.9999). To quantify the effect of c3 overexpression on sspo ( dmh4/+ ) scoliosis severity in male (E) and female (I) fish, uCT imaging was performed, followed by Cobb angle analysis (F-H, J-L). The scoliosis phenotype was not penetrant in 4 fish (Tg-male, n=1; Tg+ male, n=2; Tg+ female, n=1). These data points have been eliminated from the analysis of scoliosis severity. Scale bars = 5mm.

    Article Snippet: 2) zebrafish, p5E-elavl3 (Addgene #75025), pME-c3b.2, and p3E-polyA were recombined into pDEST Tol2 HR2 transgenesis vector.

    Techniques: Over Expression, Clone Assay, Expressing, Transgenic Assay, Quantitative RT-PCR, Imaging